Fig. 7: SERF1a enhances Htt aggregation in HD iPSC-derived neurons. | Communications Biology

Fig. 7: SERF1a enhances Htt aggregation in HD iPSC-derived neurons.

From: Amyloid modifier SERF1a interacts with polyQ-expanded huntingtin-exon 1 via helical interactions and exacerbates polyQ-induced toxicity

Fig. 7

Confocal microscopic images of iPSCs-derived neurons of normal control and HD patient infected with lentiviral EGFP construct (a) or EGFP_SERF1a construct (b). Cells were immune-stained with Htt antibody MW7. The colors for SERF1a (green), DAPI (blue), and Htt (red) are shown. The scale bars are 25 μm. (c) Calculated percentage of EGFP-positive (EGFP+) neurons harboring Htt aggregates (Htt+) in iPSC-derived neurons of normal control and patients with HD. The number of double-positive neurons was normalized to the total number of EGFP+ neurons. Only cells with Tuj1 markers were counted. EGFP C1, n = 3; EGFP GM, n = 3; EGFP_SERF1a C1, n = 19; EGFP_SERF1a GM, n = 16. Data were presented as mean ± sem and analyzed by unpaired Student’s t-test. **P < 0.01. The error bars represent SEM.

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