Fig. 4: Intracellular localization of FBP1 missense mutations in FBP1-KO HepG2 cells. | Communications Biology

Fig. 4: Intracellular localization of FBP1 missense mutations in FBP1-KO HepG2 cells.

From: Identification of genotype–biochemical phenotype correlations associated with fructose 1,6-bisphosphatase deficiency

Fig. 4

a, b Immunofluorescence studies of FBP1 missense mutants are shown. The scale bars indicate 20 µm. We counted the number of cells in two groups: diffuse localization of intracellular FBP1 and cytoplasmic aggregation of intracellular FBP1. Then, the ratio of cells with FBP1 aggregates (%) was calculated. All mutants in which hydrophobicity was unchanged, except for G294V, were diffusely localized in the cytoplasm similar, which is similar to the localization of WT FBP1. However, all mutations in which hydrophobicity was changed, except for G207R, were aggregated in the cytoplasm. The data are presented as the mean ± SD. *P < 0.05; **P < 0.01 versus WT (one-way ANOVA test followed by Dunnett’s multiple comparison test) (n = 4). c A negative correlation was observed between the number of cells with FBP1 aggregates (%) and FBP1 protein expression (mutant/WT). The correlation was determined by the Pearson coefficient of determination, R2.

Back to article page