Fig. 4: Comparison of metastatic burden and clonality in experimental and spontaneous metastasis assays in the MDA-MB-231 cell line.
From: Experimental and spontaneous metastasis assays can result in divergence in clonal architecture
a Log2-fold change in barcode number, compared to IMFP (left panel) in lungs, liver and CTCs in mice injected with barcoded MDA-MB-231 for each mode of injection. Each shape represents an independent experiment. IMFP n = 14, IMFPc n = 10, SC n = 8, ID n = 9, IV n = 8, from 2 to 3 independent experiments for MDA-MB-231 One-way ANOVA followed by Tukey multiple comparison test, *p value < 0.05, **p value < 0.005, ***p value < 0.0005, ****p value < 0.00001. Error bars represent the standard deviation of the means (SD). b Examples of barcode composition in multiple organs represented as stacked histograms for each mode of injection using MDA-MB-231 barcoded cells. c Clonal relationship between lung and liver barcode repertoires represented as scatter plots for the MDA-MB-231 model injected in the mammary fat pad (top panel) and intravenously (bottom panel). Each dot represents a barcode, and each colour represents a mouse. Fitted lines represent individual animals (IMFP n = 13, IV n = 8, from 2 to 3 independent experiments). d Pearson correlation between the top 10 barcodes found in the lungs and liver. e Pearson correlation values between barcode repertoires in different sites for the MDA-MB-231 model, according to the injection mode. f Schematic overview of clonal spread in spontaneous and experimental metastasis assays in the MDA-MB-231 model.
