Fig. 2: A comparison of ribozyme-mediated gene inactivation with RNA interference. | Communications Biology

Fig. 2: A comparison of ribozyme-mediated gene inactivation with RNA interference.

From: Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans

Fig. 2

a Confocal images of C. elegans expressing transgenic markers to label mitochondria or lysosomes in wild-type and mutant animals. Mitochondria were labeled via zjuSi47[Pcol-19>mito::mKate2] or zjuSi48[Pcol-19>tomm-20::gfp]. Lysosomes were labeled via zacSi13(Psemo-1>ctns-1-mcherry). Arrowheads: abnormally enlarged mitochondria or lysosomes. Scale bars: 5 μm. b Quantification of organelle morphology phenotypes. *p < 0.05; **p < 0.01, χ2 test. The number of animals quantified for each group was indicated above the columns. c Confocal images showing dendrite morphologies in wild-type and mutant animals. PVD dendrites were labeled via wyIs592[PVD>myr-gfp]. Scale bars: 20 μm. d Quantifications of dendrite branching phenotypes in wild-type and mutant animals. All values are presented as mean ± s.e.m. For sax-7(nj48) and sax-7::Rz: n = 21 animals; For all other genotypes: n = 20 animals. ns: not significant. *p < 0.1. ****p < 0.0001 (one-way ANOVA with the Tukey correction). Source data are provided as a Source Data file.

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