Fig. 3: The Ribo-Off system enables spatiotemporal turn-off of endogenous gene expression. | Communications Biology

Fig. 3: The Ribo-Off system enables spatiotemporal turn-off of endogenous gene expression.

From: Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans

Fig. 3

a Schematics to show the designs for achieving spatiotemporal control of Cre expression (left) and the inducible T3H38 ribozyme/Cre-loxp system to conditionally knockdown endogenous expression. b Confocal fluorescent images to show the morphology of mitochondria labeled by MITO::mKate and ARGN-1::GFP expression levels under different conditions. 2-day-old adult animals were imaged. Scale bars: 5 μm. Arrowheads: enlarged mitochondria. c Relative expression of ARGN-1::GFP under various conditions as shown in (b). All values are presented as mean ± s.e.m. Each dot represents a single animal. n = 40 for ARGN-1::GFP; n = 33 for ARGN-1::loxP::TTS::loxP::Rz without Cre; n = 43 for ARGN-1::loxP::TTS::loxP::Rz with Skin Cre; n = 36 for ARGN-1::loxP::TTS::loxP::Rz with muscle Cre; n = 32 for ARGN-1::loxP::TTS::loxP::Rz with heat-shock Cre without heat-shock; n = 39 for ARGN-1::loxP::TTS::loxP::Rz with heat-shock Cre with heat-shock. ****p < 0.0001 (one-way ANOVA with the Tukey correction). d Quantifications of the proportion of animals containing normal, intermediately defective and severely defective morphology of mitochondria. The number of animals quantified for each group was indicated above each column. Source data are provided as a Source Data file.

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