Fig. 2: miR165 and 166 act in a dose dependent manner.
From: microRNA165 and 166 modulate response of the Arabidopsis root apical meristem to salt stress
a Root meristem cell number of 5 dpg Wt root meristems exposed to 0, 100, 150, and 200 mM NaCl for 5 h (***p = 0.0010, ****p < 0.0001; One way ANOVA with post hoc Dunnett’s multiple comparison test; n = 40, 36, 34, 38). b qRT–PCR analysis of preMIR165A, preMIR166A, preMIR166B RNA levels in the root tip of Wt plants upon 0, 100, 150, and 200 mM NaCl for 5 h (*p < 0.0224, **p = 0.0078, ****p < 0.0001; two-way ANOVA with post hoc Dunnett’s multiple comparisons test; n = 3). c Root meristem cell number of 5 dpg Wt, 35S::MIM165/6 +/− (het), 35S::MIM165/6 −/− (homo) root meristems exposed to 150 mM NaCl for 5 h. (ns not significant, ****p < 0.0001 One way ANOVA with post hoc Sidak’s multiple comparison test; n = 14, 10, 16, 16, 16, 18). DIC of 5 dpg root meristems of Wt (d), 35S::MIM165/6 +/− (e), 35S::MIM165/6 −/− (f) and of Wt (g), 35S::MIM165/6 +/− (h), 35S::MIM165/6 −/− (i) root meristems exposed to 150 mM NaCl for 5 h. Scale Bar 100 μm, white arrowheads indicate the cortical stem cell, black arrowheads the TZ. Box and whiskers plots show the median, 25th and 75th percentile (box limits), the 10th and 90th percentiles (whiskers), and outliers points.
