Fig. 4: The two CDR-H3-based structural clusters differ in their HV germline usage.
From: VHH CDR-H3 conformation is determined by VH germline usage
a Top 7 most frequent VH germline usage in the dataset of unique VHH structures (n = 385) used in this study. b α101 and τ101 angle distributions in a unique set of VHH structures (n = 106) derived from the IGHV3S53 germline. c α101 and τ101 angle distributions in a unique set of VHH structures (n = 175) derived from the IGHV3-3 germline. d Top 7 most frequent VH germlines used in the combined repertoire dataset of the two sequenced llama samples. e CDR-H3 amino acid length distribution in the combined repertoire of llama 1 and 2 for antibodies either derived from IGHV3-3 (n = 13462 sequences, blue) or IGHV3S53 (n = 18769 sequences, orange) germlines (Wilcoxon rank test adjusted p-value 2e-16). f α101 and τ101 angles predicted by Alphafold for 23 VHH sequences derived from the IGHV3-3 germline (blue) and 19 VHH sequences derived from the IGHV3S53 germline (orange).
