Fig. 6: Framework 2 residue composition at positions 37 and 47 impact stability and CDR-H3 conformation.
From: VHH CDR-H3 conformation is determined by VH germline usage
a Changes in melting temperature (ΔTM) between the wildtype version of selected VHHs and their respective F37Y/F47Y mutant version. PDB code and respective chains are listed on the Y-axis. The standard deviation of three technical replicates is represented using an error bar. b Scatter plot between change in melting temperature (ΔTM) and change in CDR-H3 conformation (root-mean-square deviation; RMSD) as predicted by Omegafold for the VHH molecules shown in a.
