Fig. 2: LRO biogenetic genes are necessary for physiological and behavioral tolerance to BA toxicity.
From: Lysosome-related organelles promote stress and immune responses in C. elegans
a Experimental setup to study the involvement of LROs in protection from lethal BA toxicity after a 4-h hormetic BA preconditioning. Wild-type and LRO-deficient mutant young adult worms were subjected to a hanging drop of hormetic BA or vehicle treatment, washed, and assayed for survival upon exposure to a lethal BA dose. b Survival assays of control and BA-preconditioned wild-type and glo-1 worms exposed to a lethal dose of BA. c Fluorescence microscopy images of BA-exposed wild-type and two glo-3 mutants. d Survival assays of control and BA PC wild-type and two glo-3 mutants exposed to a lethal dose of BA. e BA-induced food avoidance of naive and BA PC wild-type and glo-1 mutants. Data are expressed as mean ± SEM. N, number of independent experiments. p-values were obtained by two-way ANOVA with Fisher’s LSD post hoc test. n.s., not significant; *p < 0.05; **p < 0.01; ***p < 0.001.
