Fig. 8: LROs are required for resistance against P. aeruginosa infection.
From: Lysosome-related organelles promote stress and immune responses in C. elegans
a, b A representative PA14 killing assay (a) and comparison of mean survivals (a) of control and BA PC wild-type and glo-1 animals. c, d A representative PA14 killing assay (c) and comparison of mean survivals (d) of control and BA PC wild-type and pgp-2 animals. Killing assays were repeated four times and compared by the log-rank (Mantel-Cox) test. Data are expressed as mean ± SEM. Detailed statistics are given in Supplementary Table 2. e, f Relative mRNA abundance of antimicrobial effector genes in wild-type, glo-1 (e), and pgp-2 (f) animals after a 16-h PA14 exposure. g, h Relative mRNA abundance of LRO-specific genes in wild-type, glo-1 (g), and pgp-2 (h) animals after a 16-h PA14 exposure. N, number of independent experiments. p-values were obtained by two-way ANOVA with Fisher’s LSD post hoc test. n.s., not significant; *p < 0.05; **p < 0.01; ***p < 0.001. i Fluorescence microscopy images of wild-type, glo-1 and pgp-2 mutants after a 16-h exposure to PA14. Scale bar for PA14 is the same as that for OP50. Every second row shows the magnified inset. Please note the gold granular fluorescent signal in the intestine of wild-type animals, and the blue-green signal in the intestinal lumen of the mutants after PA14 exposure. Representative images of three independent experiments with similar results.
