Fig. 5: Prior infection with Omicron protects hACE2.Tg mice from BA.5 challenge.
From: Omicron sub-lineage BA.5 infection results in attenuated pathology in hACE2 transgenic mice
20 hACE2 transgenic mice were given omicron (B.1.1.529) infection and were allowed to recover. After 14 days mice were divided into 4 groups: Omi-Wuhan-Hu-1 (Omicron recovered-rechallenged with Wuhan-Hu-1 strain), Omi-B.1.617.2 (Omicron recovered-rechallenged with Delta variant), Omi-B.1.1.529 (Omicron recovered-rechallenged with Omicron variant), Omi-BA.5 (Omicron recovered-rechallenged with BA.5 sub-variant) and the severity of reinfection was studied and compared with the control infected or uninfected mice. a Scheme showing the experimental design for rechallenge study. b %age change in body mass of Omi-Wuhan-Hu-1 & Omi-B.1.617.2 mice and its (c) percentage survival as compared to the Wuhan-Hu-1 or B.1.617.2 infection alone. d, e Representative images of excised lungs showing regions of inflammation & pneumonitis (black arrow) and pathological score (f) viral load in the lungs and (g) relative levels of mRNA expression of il-6 gene in the lung samples from the same group. h–m Clinical changes observed in omi-B.1.1.529 and omi-BA.5 mice as compared to B.1.1.529 or BA.5 infection alone group. h %age changes in the body mass (i) percentage survival (j and k) representative images of the excised lungs and pathological score (l) viral laod (m) relative mRNA expression of il-6 from the lung samples. For all experiment n = 5. One way-Anova using non-parametric Kruskal–Wallis test for multiple comparison. ns non-significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
