Fig. 6: Nuclease activity of BPSL1038.

a Incubation of pUC19 plasmid with rBPSL1038 shows the endonuclease sequential mechanism in nicking the plasmid by gradually transforming the supercoiled DNA to circular and linear forms. b Increasing concentrations (5, 25, 50, 100 and 200 mM) of monovalent salts (NaCl and KCl) showed gradual inhibition of rBPSL1038 nuclease activity. c Optimal nuclease activity were observed at pH 6, where at higher pH (pH 7 – 10) showed gradual inhibition, pH 4 showed precipitation. d A series of 2.5 mM divalent metal ions (Ca²⁺, Mg²⁺ and Mn²⁺) and chelating agent EDTA were tested and showed that rBPSL1038 is non-dependent towards metal ions. The images (a–d) were obtained from the original images edited using ImageJ software⁶⁵ by colour inversion using lookup table, followed by enhancing contrast with saturated pixels of 0.3% before the image were cropped. The uncropped images were shown in Supplementary Fig. 7.