Fig. 7: Interaction of double-stranded DNA (dsDNA) with BPSL1038 for metal ion independent cleavage analysis. | Communications Biology

Fig. 7: Interaction of double-stranded DNA (dsDNA) with BPSL1038 for metal ion independent cleavage analysis.

From: Structural and functional analyses of Burkholderia pseudomallei BPSL1038 reveal a Cas-2/VapD nuclease sub-family

Fig. 7

a The manual docking of dsDNA to the proposed active site of BPSL1038. b Close view of two scissile phosphates (n, n’) of the minor groove that may interact with two active sites (D11A, S33A, R30B and D11B, S33B, R30A, respectively) with the distance of ~2.6–4.5 Å, while residue R10 is proposed to interact with the phosphate group of nucleotide n + 1, n’+1. c Schematic presentation of the proposed BSPL1038-DNA interaction that may initiate metal ion-independent cleavage DNAase activity.

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