Fig. 4: Correlation of identified dSPN activity with reward rate.

a Local expression of ChRWR/Venus or rcChrimsonR-tdTomato in DMS or DLS of Tac1-Cre. Insets show the striato-nigral projections into the SNr. b Light-evoked spikes elicited by the light pulse. Latency and jittering criteria for successful identification (red symbols). c Correlation coefficient for four-channel patterns of mean spike waveforms between spontaneous and light-evoked spikes plotted against the energy of light-evoked responses for each neuron. d Responses of optogenetically identified dSPNs of DMS and DLS aligned with movement onset and outcome (reward or no-reward) tones. The yellow horizontal line represents a significant difference (p < 0.01, bin = 20 ms; t-test) between rewarded (red) and non-rewarded (blue) responses. Horizontal black bars indicate outcome tone duration. e Reward rate correlation of DMS-dSPNs with Go cue period activity during rewarded (red) and non-rewarded (blue) trials. All error bars represent SEM. On each box, the central dot indicates the median, the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers show the extreme data points not considered outliers. The histograms show r value distribution of individual neurons; colored bars represent significant correlation. The central mark on the box plot indicates the median, the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers show the extreme data points not considered outliers; p value indicates the comparison between the distribution of r values and 0 (Wilcoxon rank-sum test). f Same as e for movement onset period. g Same as e for outcome period. h Reward rate correlation of DLS-dSPNs during Go cue period. i Same as h for movement onset period. j Same as h for outcome period.