Fig. 8: Kaempferol addition results in colistin potentiation when applied to clinical strains of E. coli and A. baumannii. | Communications Biology

Fig. 8: Kaempferol addition results in colistin potentiation when applied to clinical strains of E. coli and A. baumannii.

From: Disrupting iron homeostasis can potentiate colistin activity and overcome colistin resistance mechanisms in Gram-Negative Bacteria

Fig. 8

a, b Combination of colistin with kaempferol, results in decrease of colistin MIC values for colistin-resistant E. coli K-12 strains, independent of the mechanism of colistin resistance; sensitive strains remain unaffected. WD101 is a pmrA mutant with 4-amino-4-deoxy-L-arabinose (L-Ara4N)-modified lipid A derived from strain W3110, and W3110 pDM1_mcr-1 expresses the mobile colistin resistance protein MCR-1 and, therefore bears PEtN-modifications on its lipid A. c Combination of colistin with kaempferol sensitises colistin-resistant E. coli clinical isolates. d Combination of colistin with kaempferol results in a 64-fold drop of the colistin MIC values of colistin-resistant A. baumannii clinical isolates. All assays were carried out in biological triplicate, with four technical repeats; red dotted lines indicate the EUCAST clinical breakpoint for colistin.

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