Fig. 2: GSDMC regulates apoptosis in oral keratinocytes. | Communications Biology

Fig. 2: GSDMC regulates apoptosis in oral keratinocytes.

From: METTL14-upregulated miR-6858 triggers cell apoptosis in keratinocytes of oral lichen planus through decreasing GSDMC

Fig. 2

a PI staining and quantitative analysis in HOKs transduced with control- or shGSDMC-lentivirus. b Cell death detection of HOKs transduced with control- or shGSDMC-lentivirus. c PI staining analysis and cell death measurement of HOKs with GSDMC knockdown and caspase3/7, MLKL or GSDMD knockout. d PI staining analysis and cell death measurement of HOKs with GSDMC knockdown and BAX or BAK knockout. Caspase 3 activity and proteins levels in HOKs transduced with control- or shGSDMC-lentivirus evaluated by caspase 3 activity assay (e) or western blot (f). PI staining analysis (g) and cell death measurement (h) of HOKs transduced with control- or shGSDMC-lentivirus. HOKs were pretreated with or without Q-VD-OPh (100 nM) for 4 h. ***P < 0.001 vs corresponding control group; ##P < 0.01, ###P < 0.001 vs corresponding LPS or CD4 group; n = 3; Ctrl, control; KO, knockout; dKO, double knockout. Data were expressed as means ± standard deviation. All experiments were carried out at least 3 times. Student’s t test (a, b, e) and one-way ANOVA (c, d, g, h) were used for statistical analysis.

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