Table 1 Structural parameters determined by SEC-UV-SAXS for MmSerB2 dimer, MaSerB dimer, MtSerB2 dimer, MtSerB2 trimer and MtSerB2 tetramer.
MmSerB2 dimer | MaSerB dimer | MtSerB2 dimer | MtSerB2 trimer | MtSerB2 tetramer | |
|---|---|---|---|---|---|
Guinier analysis | |||||
I(0) (cm−1) | 0.1017 ± 5.736 10−5 | 0.1096 ± 5.991 10−5 | 0.0937 ± 3.322 10−5 | 0.0422 ± 7.024 10−5 | 0.0578 ± 5.299 10−5 |
Rg (Å) | 32.32 ± 0.03 | 32.62 ± 0.03 | 32.47 ± 0.02 | 39.61 ± 0.16 | 39.89 ± 0.06 |
qmin (Å−1) | 0.00365 (n = 1) | 0.00365 (n = 1) | 0.00365 (n = 1) | 0.00502 (n = 4) | 0.00365 (n = 1) |
qRg max | 1.2972 | 1.2942 | 1.3032 | 1.0116 | 1.2919 |
Coefficient of correlation, R2 | 0.9952 | 0.9992 | 0.9989 | 0.9948 | 0.9901 |
P(r) analysis | |||||
I(0) (cm−1) | 0.1022 ± 4.84 10−5 | 0.1101 ± 5.25 10−5 | 0.0938 ± 2.93 10−5 | 0.0422 ± 6.21 10−5 | 0.06 ± 4.34 10−5 |
Rg (Å) | 32.54 ± 0.02 | 32.84 ± 0.02 | 32.55 ± 0.02 | 39.94 ± 0.07 | 39.62 ± 0.04 |
Dmax (Å) | 99 | 101 | 107 | 143 | 125 |
χ2 (total estimate from GNOM) | 1.134 (0.902) | 1.245 (0.900) | 1.183 (0.831) | 0.909 (0.868) | 1.244 (0.976) |
Molecular weight analysis | |||||
Vp (nm3) | 125 | 126 | 127 | 183 | 254 |
Vp MW (kDa) | 103.7 | 104.7 | 105.5 | 152.2 | 210.7 |
Bayesian inference MW (conf. interval) (kDa) | 91.2 (84.3–95.8) | 91.2 (84.3–95.8) | 88.3 (84.3–95.8) | 130.9 (121.5–142.2) | 185.8 (162.7– 221.1) |
