Fig. 1: MET silencing in MET-dependent cells triggers striking morphological changes.
From: Aberrant MET activation impairs perinuclear actin cap organization with YAP1 cytosolic relocation
a Protein analysis of MET signaling pathway in LoVo control (MET + ) and LoVo MET-KO cells. GAPDH was used as loading control. b LoVo MET+ and MET-KO stained with pMET (green) and Phalloidin (red) and counterstained with DAPI (blue). Scale bar: 50 μm. c Evaluation of cell proliferation over a time-lapse of 48 h in LoVo MET+ and MET-KO by means of Phasefocus LiveCyteTM platform. Pictures were taken every 4 h. Data were normalized on cell count at T0 and shown as mean ± SEM between two independent experiments. A total of 800 cells was analyzed in each experiment. d LoVo MET+ and MET-KO morphology visualized with phase contrast microscopy. Scale bar: 100 μm. e–h Measurement of cell area, perimeter, length/width ratio and sphericity of LoVo MET+ and MET-KO in a 48 h time-lapse performed with Phasefocus LiveCyteTM. Pictures were taken every 4 h. Median and quartiles distribution are plotted. Outliers were identified and cleaned from results by means of ROUT method (Q = 1%). A total of 800 cells was analyzed. Statistic was calculated by T-test.
