Fig. 2: Evaluation of fatty acid elongases for production of nervonic acid. | Communications Biology

Fig. 2: Evaluation of fatty acid elongases for production of nervonic acid.

From: High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering

Fig. 2

A Expression of CgKCS in Y. lipolytica po1g. B The control strain po1g. C Expression of AtFAE1 in po1g. D Expression of BtFAE1 in po1g. E Co-expression of AtFAE1, BtFAE1 and CgKCS in po1g. F Diagram of fatty acid metabolism related to multiple organelles and subcellular localization of CgKCS. ACL, ATP-citrate lyase; ACC, acetyl-CoA carboxylase; CgKCS, β-ketoacyl-CoA synthase of C. graeca; FAS, fatty acid synthase; FFA, free fatty acids; PalCoA, palmitoyl-CoA. G The relative amount of nervonic acid content in the TFA produced by the strains expressing CgKCS and CgKCS fused with organelle targeting signals. Cultivations were carried out for 144 h at 250 rpm and 28 °C in flasks. Data are mean ± s.d. from three replicates. H Microscopic observation of the strains expressing the fused protein CgKCS-sfGFP for evaluating the location of CgKCS in Y. lipolytica. CgKCS-sfGFP exhibited both punctate and accumulated localization at the early stage of cultivation (36 h) and gathered in lipid droplets at lipid accumulation stage (60 h).

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