Fig. 4: Construction of VLCFAs-producing strains expressing CgKCS, gELOVL6 and MaOLE2 by random insertion and homologous recombination. | Communications Biology

Fig. 4: Construction of VLCFAs-producing strains expressing CgKCS, gELOVL6 and MaOLE2 by random insertion and homologous recombination.

From: High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering

Fig. 4

A Schematic diagram showing the construction of YLVL strains. The overexpressed genes are indicated by red symbol. The red numbers in parentheses represented the gene copies more than one on plasmids. The green italics symbol denotes genes integrated into the genomes by random integration or homologous recombination. The results of lipid and VLCFAs biosynthesis in the strains YLVL3, 6, 7, 8 and 10, including the fatty acid profiles (B), the titer of lipid and nervonic acid (C), the fatty acid composition in YLVL10 detected by gas chromatography (D), the titer of LCFAs and VLCFAs (E), and the fraction of LCFAs and VLCFAs (F). The yeasts were cultured for 144 h at 250 rpm and 28 °C in flasks. Data are mean ± s.d. from three replicates. Statistically significant differences between each engineered Y. lipolytica strains were denoted *P < 0.05, **P < 0.01 (two-tailed Student’s t-test).

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