Fig. 5: The construction of nervonic acid overproducing strains by homologous recombination. | Communications Biology

Fig. 5: The construction of nervonic acid overproducing strains by homologous recombination.

From: High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering

Fig. 5

A Schematic diagram showing the construction of YLNA strains by expressing the genes CgKCS, MaOLE2, YlINO2 and MoGPAT-88 at multiple genomic loci. The overexpressed genes are indicated by blue symbol. The blue numbers in parentheses represented the gene copies more than one on plasmids. The green italics symbol denotes genes integrated into the genomes by homologous recombination. B Comparison of the production of nervonic acid and lignoceric acid in YLNA9, YLNA10 and YLVL10. The results of lipid and VLCFAs biosynthesis in the strains YLNA1, 3, and 5 to 10, including the fatty acid profiles (C), the titer of lipid and nervonic acid (D), the fatty acid composition in YLNA10 detected by gas chromatography (E). The yeasts were cultured for 144 h at 250 rpm and 28 °C in flasks. For YLNA1-YLNA6 in (C, D), n = 3; For YLNA7-YLNA10 in (C, D), n = 2. Statistically significant differences between each engineered Y. lipolytica strains were denoted *P < 0.05, **P < 0.01 (two-tailed Student’s t-test).

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