Fig. 8: Nervonic acid and lipids improved by overexpressing GPAT and DGAT2 from M. oleifera.

A The biosynthesis of TAG in ER catalyzed by GPAT, LPAT, PAP and DGAT. G3P glycerol 3-phosphate, GPAT glycerol-3-phosphate acyltransferase, LPAT lysophosphlipid acyltransferase, PAP phosphatidic acid phosphatase, DGAT diacylglycerol acyltransferase. Phylogenetic trees of the acyltransferases GPAT (B) and DGAT2 (C) from M. oleifera constructed by the Neighbor-Joining method using the software MEGA11. The titer of lipid and nervonic acid in the acyltransferases overexpressed strains (D). The fatty acid composition in GPAT (E) and DGAT2 (F) overexpressed strains.