Fig. 9: Fed-batch fermentation in a 50-L reactor.

A Conditions for controlling the two-stage fermentation process. B Fed-batch fermentation of YLVL10 in a 50-L bioreactor. C Fed-batch fermentation of YLNA9 in a 50-L bioreactor. Fermentation was conducted at 28 °C, pH 5.5. Substrate feeding to 150 g/L in every 12 h. DCW, dry cell weight.