Fig. 2: oPR software workflow and graphical user interface (GUI) for programming oPR protocols.
From: High-throughput feedback-enabled optogenetic stimulation and spectroscopy in microwell plates
a Software architecture for communication between user, computer, and oPR. The GUI and Protocol Python scripts send user-defined stimulation and measurement protocols to the optoPlate and optoReader Arduinos that control individual components in both device modules. Photodiode measurements collected by the optoReader Arduino are returned to the computer for reporting or feedback-driven signal adjustments. b Windows of the GUI. (1) The user can automatically calibrate OD and Blue LEDs from the homescreen. (2) Wells, light dose, illumination timing, and feedback algorithm are specified for up to 96 independent protocols. (3) Photodiode reading frequency parameters are specified. (4) The user can review and save their experimental protocols prior to running the experiment.
