Fig. 5: LysM homologs assist the interaction between VgrG and the downstream effector. | Communications Biology

Fig. 5: LysM homologs assist the interaction between VgrG and the downstream effector.

From: Genome wide analysis revealed conserved domains involved in the effector discrimination of bacterial type VI secretion system

Fig. 5

a The vgrG loci of Ketobacter alkanivorans GI5 and Burkholderia sp. RPE67. b Immunoblots demonstrating the expression of VgrG2bG15, LysMG15 and Kalk_10455 in E. coli. Anti-RpoB is lysis control. c Survival of E. coli expressing VgrGG15, LysMG15 and Kalk_10455 in pETduet. Ten-fold serial dilutions of cultures were spotted on LB agar containing the stated concentrations of IPTG and grown for 24 h. The image is representative of three independent experiments. d Interactions between VgrGG15, LysMG15 and Kalk_10455. Shown here are immunoblots of lysates (total) and immunoprecipitates with anti-FLAG affinity beads (IP: FLAG) of Kalk_10455 and GFP transformed with a plasmid encoding Myc-tagged VgrGG15 or Strep-tagged LysMG15. 0423PA is control protein. e Immunoblots demonstrating the expression of BRPE_05220 and NLPC_P60 domain in E. coli. Anti-RpoB is lysis control. f Survival of E. coli expressing BRPE_05220 and NLPC_P60RPE domain in pETduet. Ten-fold serial dilutions of cultures were spotted on LB agar containing the stated concentrations of IPTG and grown for 24 h. The image is representative of three independent experiments. g LysM domain mediates the interaction between VgrGRPE and BRPE_05220. Shown here are immunoblots of lysates (Input) and immunoprecipitates with an anti-FLAG affinity beads (IP:FLAG) of BRPE_05220 or BRPE_05220ΔLysM transformed with a plasmid encoding either Myc-tagged VgrGRPE or Myc-tagged 0423PA. 0423PA is control protein.

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