Fig. 3: Taxonomic assignments of soil metatranscriptomes using soil invertebrate genomes.

a Assignment success of soil metatranscriptomic reads using genomes available in NCBI RefSeq (white) and MetaInvert genomes in addition to NCBI genomes (grey). Please note the log-scale of the y-axis (taxon observations with RefSeq genomes: Collembola n = 290, Myriapoda n = 80, Oribatida n = 90; independent taxon observations with RefSeq + MetaInvert genomes: Collembola n = 810, Myriapoda n = 270, Oribatida n = 630), center line: median; box limits: upper and lower quartiles; whiskers: 1.5× interquartile range; points: outliers; b reads assigned to common soil invertebrate classes, with NA marking metazoan reads not assigned to soil invertebrates at the class level; c soil invertebrate richness trend along an elevation gradient (grey area marks standard error of the trendline). Assignments are available as Supplementary Data 2.