Fig. 3: Bortezomib enhances capsaicin-evoked currents.
From: Bortezomib-induced neuropathy is in part mediated by the sensitization of TRPV1 channels
a Left, voltage clamp traces of current evoked by perfusion of 1 µM capsaicin from a naive mouse DRG neuron and a neuron treated with 100 nM bortezomib for 24 h. These cells are also capsaicin-naïve prior to their 1 µM dose of capsaicin. Right, capsaicin evoked current density in naïve (black, 44 ± 9 pA/pF, n = 16) and bortezomib treated DRG neurons (red, 81 ± 11 pA/pF, n = 19, p < 0.05, Student’s t test, t = 2.605, df = 33). b Left, resting membrane potential in naïve and bortezomib treated capsaicin sensitive (TRPV1+) mouse DRG neurons (n = 14–20 for each group, p > 0.05 for all). Middle, minimum (threshold) current injection necessary to elicit an action potential in naïve and bortezomib treated capsaicin sensitive (TRPV1+) mouse DRG neurons (n = 14–20 for each group, p > 0.05 for all). Right, firing rate (Hz) in response to a 2 nA ramp current injection stimulus in naïve and bortezomib treated capsaicin sensitive (TRPV1+) mouse DRG neurons (n = 14–20 for each group, p > 0.05 for all). Data presented as mean ± SEM. Dots represent individual cells.
