Fig. 4: Bortezomib-induced neurotoxicity.
From: Bortezomib-induced neuropathy is in part mediated by the sensitization of TRPV1 channels
a Representative images of primary DRG neurons stained with Tuj1 after treatment with DMSO or Bortezomib (30 nM). Right, dose response curve of bortezomib action on neurite outgrowth measured by well indicating significant loss of neurites after 24 h of treatment. p < 0.05 compared to DMSO, one-way ANOVA followed by Dunnett’s multiple comparisons test, F8, 37 = 20.58. b Decrease in average neurite outgrowth per neuron, number of branches per neurite and number of processes per neuron with bortezomib treatment. *, p < 0.05, Student’s T test (neurite outgrowth: t = 5.468, df = 34; number of branches: t = 5.131, df = 34; number of processes: t = 2.595, df = 34). c Number of neurons estimated after incubation with DMSO or bortezomib 30 mM. *, p < 0.05, Student’s T test (t = 3.486, df = 34). d Mean neurite outgrowth per neuron from TRPV1 KO mice treated with DMSO or bortezomib 30 mM. *, Scale bar: 100 microns. Data presented as mean ± SEM. Dots represent individual neurons.
