Fig. 4: Flow diagram showing the setup for studying peristalsis. | Communications Biology

Fig. 4: Flow diagram showing the setup for studying peristalsis.

From: Advancing peristalsis deciphering in mouse small intestine by multi-parameter tracking

Fig. 4

The setup involves tissue bath, with units for pressure transducer-amplifier, camera control and droplet discharge sensor-meter. The setup allows for a comprehensive study of intestinal motility capturing movements of the intestinal segment mounted in a perfusion bath using a Keyence camera, multi-spectrum LumiTrax light (MSL) and CV-X400 series controller system. Changes in intraluminal pressure were measured using a pressure transducer and transducer amplifier module (TAM-A) connected to the ports on the tissue bath. A droplet discharge occurring at the aboral end was detected using the infrared-LED sensor drop counter (Vernier Instruments, USA) and metered using LabQuest Mini Interface. Together this setup allows for a comprehensive study of intestinal motility and enhances the understanding of complex biological processes responsible for droplet discharge. The figure was created using Corel Draw, version 21.3.0.755 (2019).

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