Fig. 6
From: Advancing peristalsis deciphering in mouse small intestine by multi-parameter tracking
Panel graphs showing traces of factors responsible for droplet discharge duration and segmentation: a SD discharge: in BPhase to NPhase, longitudinal muscles showed maximum values, EW1 and EW4 begins to contract with maximum reduction occurring in the DPhase. Longitudinal muscle contraction at proximal end occurred before circular muscle began to contract. EW1g showed greater contraction when compared to EW4g. Correspondingly, maximum increase in volume was seen in BPhase and thereafter, the volume began to decrease with maximum reduction in the middle of DPhase. Lg, EWg and Vg showed reduction in the DPhase, where Ps was increased. Lg increased from the middle of DPhase and reached a maximum toward the early part of APhase while, EWg and Vg continued to increase through the APhase. Ps decreased during the APhase. b LA discharge: longitudinal muscles showed an increase from BPhase to DPhase and thereafter decreased from the beginning of DPhase and continued into the APhase. EW1 started to contract in BPhase, NPhase and early DPhase, while longitudinal muscle remained in the contracted state at the proximal end. EW1g showed a marginal increase in DPhase and APhase. Unlike EW1g, EW4g contractions were minimal. Volume changes are minimal compared to SD. An increase in volume was observed from NPhase to DPhase, followed by a decrease in APhase. c LD discharge: longitudinal muscle contraction showed a decrease in BPhase to NPhase, unlike SD and LA. EW1g values increased from BPhase to DPhase, suggesting continued filling through BPhase and NPhase, and began to empty only in the DPhase. Unlike SD and LA, in LD, the lumen continued to fill through BPhase and NPhase. EW4g continued to decrease from BPhase to APhase with maximum decrease occurring in BPhase to NPhase. Volume decreased in DPhase and did not return to base levels suggesting incomplete emptying. d Diameter changes between EW1 and EW4 depicting anterograde and retrograde contraction analyzed using “GutCode”. EW1 contraction with EW4 relaxation causes anterograde movement (upward arrow), and EW1 contraction with EW4 relaxation causes retrograde contraction (downward arrow). e Diameter changes between EW1 and EW4 depicting segmental contractions analysed by “GutCode”. Simultaneous contraction or relaxation of EW1 and EW4 causes pure segmental contraction. A small increase in diameter of EW4 compared to EW1 causes segmental forward, and a small decrease in diameter of EW4 compared to EW1 causes segmental backward contractions.
