Fig. 1: Atg7 interacts with PDCD4 and negatively regulates PDCD4 protein levels.

a, b Co-immunoprecipitation assay was performed to detect the interaction between Atg7 and PDCD4. Lysates were extracted for immunoprecipitation with Atg7 (A) antibody or PDCD4 (B) antibody. c Glutathione S-transferase (GST) or GST-fused Atg7 protein and in vitro translated 3*Flag-PDCD4 were incubated for four hours, and the reaction was analyzed by SDS-PAGE. d Glutathione S-transferase (GST) or GST fused PDCD4 protein and in vitro translated 3*Flag-Atg7 were incubated for four hours, and the reaction was analyzed by SDS-PAGE. e Co-transfected GFP-Atg7 and 3*Flag-PDCD4 plasmids in HEK293 cells, followed by immunofluorescence experiments. The Pearson’s correlation and overlap coefficient are shown in bar graph format (n = 20 cells) from three independent experiments were analyzed. f PDCD4 expression levels were increased in HCT116 Atg7-KO cells compared with that in Atg7-WT cells. Data represent the mean ± SD of three independent experiments (n = 3). P values were calculated by t test. *P < 0.05. g Interaction between endogenous Atg7 and PDCD4 in HCT116 cells under serum starvation for four hours. h Interaction between endogenous Atg7 and PDCD4 in HCT116 cells under ATP depletion induced by 2DG (5 mM) and oligomycin (2.5 μM) treatment for four hours.