Table 1 Cryo-EM data collection, refinement, and validation statistics.
 | mD2VLP-K8b-IgG1 immunocomplex (EMDB-36408) |
---|---|
Data collection and processing | |
 Magnification | 15,000 |
 Voltage (kV) | 200 |
 Electron exposure (e–/Å2) | 32 |
 Defocus range (μm) | 2.3–4.2 |
 Pixel size (Å) | 4 |
 Symmetry imposed | icos |
 Initial particle images (n) | 2035 |
 Final particle images (n) | 1246 |
 Map resolution (Å)  FSC threshold | 16.3 0.143 |
 Map resolution range (Å) | 16.0–19.9 |
Refinement | |
 Initial model used (PDB code) | n.a. |
 Model resolution (Å)  FSC threshold | n.a. |
 Model resolution range (Å) | n.a. |
 Map sharpening B factor (Å2) | n.a. |
 Model composition  Non-hydrogen atoms  Protein residues  Ligands | n.a. |
B factors (Å2)  Protein  Ligand | n.a. |
RMS deviations  Bond lengths (Å)  Bond angles (°) | n.a. |
Validation  MolProbity score  Clashscore  Poor rotamers (%) | n.a. |
Ramachandran plot  Favored (%)  Allowed (%)  Disallowed (%) | n.a. |