Table 1 Cryo-EM data collection, refinement, and validation statistics.

From: Antibodies from dengue patients with prior exposure to Japanese encephalitis virus are broadly neutralizing against Zika virus

 

mD2VLP-K8b-IgG1 immunocomplex (EMDB-36408)

Data collection and processing

 Magnification

15,000

 Voltage (kV)

200

 Electron exposure (e–/Å2)

32

 Defocus range (μm)

2.3–4.2

 Pixel size (Å)

4

 Symmetry imposed

icos

 Initial particle images (n)

2035

 Final particle images (n)

1246

 Map resolution (Å)

 FSC threshold

16.3

0.143

 Map resolution range (Å)

16.0–19.9

Refinement

 Initial model used (PDB code)

n.a.

 Model resolution (Å)

 FSC threshold

n.a.

 Model resolution range (Å)

n.a.

 Map sharpening B factor (Å2)

n.a.

 Model composition

 Non-hydrogen atoms

 Protein residues

 Ligands

n.a.

B factors (Ã…2)

 Protein

 Ligand

n.a.

RMS deviations

 Bond lengths (Å)

 Bond angles (°)

n.a.

Validation

 MolProbity score

 Clashscore

 Poor rotamers (%)

n.a.

Ramachandran plot

 Favored (%)

 Allowed (%)

 Disallowed (%)

n.a.

  1. Refinement was performed by EMAN2 software. The solved cryo-EM structure had suboptimal resolution (16.0–19.9 Å); thus, the requested detailed structural parameters could not be provided with accuracy.
  2. n.a. answers were not available.