Table 1 Cryo-EM data collection and validation.
| Â | Overlapping tri-nucleosome (PDB: 8IHL, EMDB: EMD-35448) |
|---|---|
Data collection and processing | Â |
Microscopy | Krios G3i |
Detector | K3 BioQuantum |
Magnification | 81,000 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 55.1 |
Defocus range (µm) | 1–2.5 |
Pixel size (Ã…) | 1.07 |
Symmetry imposed | C1 |
Initial particle (no.) | 1,718,300 |
Final particle (no.) | 148,082 |
Map resolution (Å) (FSC = 0.143) | 7.6 |
Map resolution range (Å) | 5.8–9.9 |
Map sharpening B factor (Å2) | −364.5 |
Refinement | Â |
Initial model used (PDB code) | 5GSE |
Model composition | Â |
Protein residues | 1728 |
Nucleotide residues | 706 |
R.m.s. deviations | Â |
Bond lengths (Ã…) | 0.004 |
Bond angles (degree) | 0.803 |
MolProbity score | 1.75 |
Clashscore | 17.76 |
Poor rotamers (%) | 0.00 |
Ramachandran plot | Â |
Favored (%) | 99.17 |
Allowed (%) | 0.83 |
Disallowed (%) | 0 |
Q-score (all chains) | 0.109 |
