Fig. 2: Two activated mutations of AR, Q62L and E81Q obviously induce the hepatoma cell growth and proliferation. | Communications Biology

Fig. 2: Two activated mutations of AR, Q62L and E81Q obviously induce the hepatoma cell growth and proliferation.

From: Two somatic mutations in the androgen receptor N-terminal domain are oncogenic drivers in hepatocellular carcinoma

Fig. 2

a, b Shown was the stable expression of AR WT, AR Q62L and E81Q in MHCC-97H cells. The mRNA level (a) and the protein level (b) were shown. mRNA data was detected by qRT-PCR. Data (mean ± SD, n = 3) was normalized by GAPDH and analyzed by one-way ANOVA; ***p < 0.001. Protein data was detected by Western Blot and TUBULIN was analyzed as a control. c, d Two N-term active somatic mutations (Q62L/E81Q) of AR induce cell clone formation function in MHCC-97H stable expression cell line. The colonies of 1000 cells of different group after 14 days were shown. Data (mean ± SD, n = 3) was analyzed by one-way ANOVA; *p < 0.01; **p < 0.001. (Scale bar = 1 cm). e Two N-term active somatic mutations (Q62L/E81Q) of AR induce cell growth and proliferation function in MHCC-97H stable expression cell line. The cell growth function among 6 days was detected by CCK-8 were shown. Data (mean ± SD, n = 3) was analyzed by one-way ANOVA; **p < 0.001; ***p < 0.001.

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