Fig. 3: Effect of SH3RF2 depletion on DDP resistance in OC in vitro.

a DDP-resistant OC cells with stable depletion of SH3RF2 were established and the expression of SH3RF2 was evaluated by RT-qPCR (n = 3) and western blotting (n = 3). b DDP-resistant OC cells with or without depletion of SH3RF2 were exposed to 0, 2.5, 5, 10, 20, 40, or 80 μM DDP for 48 h and then MTT assays were performed (p < 0.01 vs. the shNC group). n = 3. The IC50 values of DDP in parental and DDP-resistant OC cells were calculated and SH3RF2 depletion significantly decreased IC50 values of DDP in DDP-resistant OC cells. n = 3. DDP-resistant OC cells with or without depletion of SH3RF2 were exposed to DDP (A2780/DDP: 30 μM; SKOV3/DDP: 22 μM) for 48 h. c Cell apoptosis were determined by flow cytometry following Annexin V-FITC/PI double staining. n = 3. d Cell apoptosis were detected by AO/EB staining (Scale bars, 100 μm). n = 3. e Western blots of lysates from DDP-resistant OC cells stained for cleaved PARP and cleaved caspase 3. n = 3. f Caspase 3 activities were measured by the commercial assay kits. n = 3. g Cell proliferation was determined by colony formation assay. n = 3. Data are expressed as the mean ± SD. The p values were determined by one-way or two-way ANOVA. NS, no significance.