Fig. 1: Purification and characterization of GS from methanogenic archaea. | Communications Biology

Fig. 1: Purification and characterization of GS from methanogenic archaea.

From: Differences in regulation mechanisms of glutamine synthetases from methanogenic archaea unveiled by structural investigations

Fig. 1

a SDS-PAGE and (b) Native PAGE of 2 µg purified MtGS and MsGS. c Relative activity of MtGS at different 2OG concentrations. The presented fit (grey plain line) is extrapolated from the determined Michaelis-Menten parameters. d The specific activity of MsGS without or with 2 mM 2OG at 40 mM glutamate and 20 mM NH4Cl. For c and d data is represented as mean (by cyan diamonds on panel (c)) ± standard deviation (s.d.) and individual values are shown as white circles (n = 3).

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