Table 1 Kinetic parameters of MtGS and MsGS for NH4Cl and glutamate.

From: Differences in regulation mechanisms of glutamine synthetases from methanogenic archaea unveiled by structural investigations

Enzyme

Substrate

AppKm (mM)

AppVmax (U.mg−1)

Hill coefficient

MtGS

NH4Cl

0.14 ± 0.01

3.269 ± 0.115

1.48 ± 0.21

Glutamate

8.76 ± 0.87

3.419 ± 0.134

1.69 ± 0.23

MsGS

NH4Cl

0.07 ± 0.01

0.98 ± 0.02

0.84 ± 0.12

Glutamate

32.57 ± 3.13

1.40 ± 0.07

2.35 ± 0.41

  1. All activities were measured in technical triplicates (n = 3). MtGS was measured in the presence of 2 mM 2OG. The curves used for kinetics parameter determination are provided in Fig. S5.
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