Fig. 3: Impact of stromal cells on myeloid cell-secreted cytokines. | Communications Biology

Fig. 3: Impact of stromal cells on myeloid cell-secreted cytokines.

From: Single-cell analysis reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of ovarian cancer

Fig. 3

a Average expression of selected cytokines and SPs in each cell type and tumor group. Dot plot showing the mean gene expression z-score of differentially expressed cytokines and SPs across different cell types. The expression of each gene is normalized to a mean value of zero and unit standard deviation. Expression values are indicated by color. Adjusted p-values for each differentially expressed gene between high- and low-groups are indicated by circle size. b Violin plots showing the expression of CXCL9 and CXCL10 in macrophages and dendritic cells (DCs). c The expression of CXCL1, CXCL5, IL1A, and NFKB1 in macrophages, and d the expression of CXCL8 and NFKB1 in DCs by high-/low-stromal group. P-values are computed from the two-sided Wilcoxon test, adjusted based on Bonferroni correction using all genes in the dataset. Statistical significance is coded by the following symbols. p-value < 0.1, *p-value < 0.05, **p-value < 0.01, and ***p-value < 0.001. e Top, human cytokine arrays used macrophage supernatants cultured with mesenchymal stem cells (MSC), tumor cells (TC), or MSC/TC conditioned media as described in Material and Methods. f Bar plot summarizing relative intensities of cytokines with the greatest increase, plotted in Image J.

Back to article page