Fig. 6: Spatial distribution of CXCL12, CD8+ T cells, and NK cells in high- and low-stromal OvCa tissues. | Communications Biology

Fig. 6: Spatial distribution of CXCL12, CD8+ T cells, and NK cells in high- and low-stromal OvCa tissues.

From: Single-cell analysis reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of ovarian cancer

Fig. 6

a Box plot showing the expression of selected marker genes by sample. Based on the average expression of COL1A1, the samples were split into high-stromal (blue) or low-stromal (yellow) groups. b The spatial autocorrelation of selected cytokines, SPs, and marker genes by sample. Moran’s I test, implemented in the Seurat FindSpatiallyVariableFeatures function, was applied to compute the autocorrelation between the expression of each gene and its spatial location. c Representative spatial feature plots showing mRNA levels of stromal markers (COL1A1, ACTA2, EPCAM), CXCL12, CD8+ T cell marker (CD8A) and NK marker (KLRB1) from two high-stromal samples d and two low-stromal samples. Arrows indicate regions COL1A1, ACTA2 and CXCL12. e Heatmap showing the Spearman correlation coefficients between the expression of COL1A1/ACTA2 and CXCL12. f Representative image of CD8 (brown) and CXCL12 (magenta) double stained in a paraffin-embedded human OvCa tissue section. The scale bar equals 100 mm.

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