Fig. 8: Changes in lipidome in the Mppgdh mutants.

a, b PCA score plots of male (a) and female (b) 14-day thallus samples grown under L/D, L/D + serine, and CL conditions (n = 3). c, d Venn diagrams showing the significantly decreased (c) and increased (d) lipid classes in thalli of Mppgdh-1 and Mppgdh-3 under L/D and CL conditions. e PCA score plot of the antheridial receptacle samples grown under L/D conditions (n = 4). f Volcano plot showing the DALCs in antheridial receptacles of Mppgdh-1. Red dots and blue squares represent significantly increased (p-value < 0.05, fold change > 2) and decreased (p-value < 0.05, fold change < 0.5) lipid classes, respectively, in Mppgdh-1. Black triangles represent no significant differences between Tak-1 and Mppgdh-1. TG triacylglycerol, OxTG oxidized triglyceride, LPG lysophosphatidylglycerol, LPC lysophophatidylcholine, PE phosphatidylethanolamine, LPE lysophosphatidylethanolamine, LDGTS lysodiacylglyceryl trimethylhomoserine/Lysodiacylglyceryl hydroxymethyl-N,N,N-trimethyl-β-alanine, OxFA oxidized fatty acid, CoQ coenzyme Q, DGGA diacylglyceryl glucuronide, STSE stigmasterol ester, SISE sitosterol ester, BRSE brassicasterol ester, CASE campesterol ester, MG monoacylglycerol, PhytoSph phytosphingosine, OxPC oxidized phosphatidylcholine.