Fig. 2: Next-generation sequencing following SECRETS screens reveals candidate x-gRNAs for EMX1 with high activity and specificity. | Communications Biology

Fig. 2: Next-generation sequencing following SECRETS screens reveals candidate x-gRNAs for EMX1 with high activity and specificity.

From: Selection of extended CRISPR RNAs with enhanced targeting and specificity

Fig. 2

The average fraction of next-generation sequencing (NGS) reads from two SECRETS replicates reveals several standout x-gRNAs from E. coli that survived (Ex1-5). These top 5 (red dots) were subsequently evaluated for subsequent in vitro validation.

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