Fig. 4: SECRETS can identify high-specificity x-gRNAs that outperform eCas9.
From: Selection of extended CRISPR RNAs with enhanced targeting and specificity
a Highly specific x-gRNAs could also be readily identified from SECRETS screens for gRNAs for FANCF and b VEGFA targets. Note significant off-target activity even with eCas9 with the VEGFA gRNA that was blocked by the Vx4-gRNA, with comparable on-target activity, and FANCF x-gRNAs have no detectable off-target activity. n = (at least) 3 replicates each, dots are individual data points. Error bars = standard error of the mean. eCas9 = enhanced specificity Cas9. See the activities of the full set of the top 4 FANCF and VEGFA x-gRNAs in Fig. S3.
