Fig. 6: CBN effect on apparent conductance compared to its effect on inactivation.
a Comparison of concentration-dependent effects of CBN on Gmax* vs. inactivation as a percentage of vehicle. b Normalized relationship of the data from a and fit with the Hill equation. The Hill coefficient was not constrained during fitting. c Cartoon representation of the concentration-dependent modality of CBN’s modulation of voltage-dependent Nav currents. Given than CBN is hydrophobic, it readily partitions into the membrane. Once inside the membrane it interacts to a very small extent with the (1) resting state and a much larger affinity for the (2) inactivated states of the Nav channel. (3) CBN equipotently prevents channels from opening, as it hyperpolarizes/enhances inactivation hence.
