Table 1 Data collection, phasing and refinement statistics.

PDB accession

8B46

7Z8Y

8B5X

Space group

P6₃

P6₃

P6₃

   a, b, c (Å)

134.09, 134.09, 106.59

133.76, 133.76, 106.71

133.92, 133.92, 106.58

   α, β, γ (°)

90, 90, 120

90, 90, 120

90, 90, 120

Resolution (Å)

116.13–1.67 (1.89–1.67)^a

78.49–2.29 (2.52–2.29)^a

115.98–1.98 (2.22–1.98)^a

Ellipsoidal diffraction limits (Å) with principal axes

2.21 (1, 0, 0) 0.894 a^a - 0.447 b^a 2.21 (0, 1, 0) b^a 1.63 (0, 0, 1) c^a

2.79 (1, 0, 0) 0.894 a^a - 0.447 b^a 2.79 (0, 1, 0) b^a 2.22 (0, 0, 1) c^a

2.60 (1, 0,0) 0.894 a^a - 0.447 b^a 2.60 (0, 1, 0) b^a 1.92 (0, 0, 1) c^a

R_meas

0.140 (1.987)

0.333 (2.253)

0.221 (2.150)

R_pim

0.043 (0.622)

0.074 (0.499)

0.048 (0.458)

I / σ(I)

17.4 (1.9)

10.6 (1.8)

12.5 (1.8)

CC_1/2

0.999 (0.676)

0.995 (0.643)

0.998 (0.663)

Completeness (spherical) (%)

55.7 (8.9)

67.2 (13.6)

57.8 (10.1)

Completeness (ellipsoidal) (%)

96.1 (76.7)

95.2 (77.8)

95.3 (79.5)

Redundancy

21.1 (20.4)

20.0 (20.4)

21.2 (22.1)

Resolution (Å)

32.21 – 1.67

43.78–2.29

32.17–1.98

No. reflections

70727

32659

43339

   R_work / R_free

0.1658/0.1896

0.1847/0.2183

0.1733/0.2108

   Cruickshank DPI (Å)

0.2

0.2

0.2

No. atoms

5547

5192

5275

   Protein

4961

4960

4939

   Ligand/ion

4

4

4

   Water

582

228

332

B-factors

40.52

46.62

45.50

   Protein

40.10

46.84

45.63

   Ligand/ion

27.57

35.65

52.63

   Water

44.26

42.10

43.40

   Bond lengths (Å)

0.014

0.004

0.008

   Bond angles (°)

1.296

0.731

1.096