Fig. 3: CST alleviated the progression of femoral-head necrosis in vivo. | Communications Biology

Fig. 3: CST alleviated the progression of femoral-head necrosis in vivo.

From: Cortistatin prevents glucocorticoid-associated osteonecrosis of the femoral head via the GHSR1a/Akt pathway

Fig. 3

a Representative images of H&E stain of different indicated processing groups. Scale bars, 100 μm (low field), 50 μm (high field). b The statistical analysis of empty lacunae ratio of 1. The Blank group. 2. The CST group. 3. The MPS + PBS group (as a control group). 4. The MPS + CST group. 5. The MPS + CST + MK2206 group. c, d Immunohistochemical assay of osteocalcin in the femoral head of the indicated group. e The representative images of TRAP stain of different groups. The red arrow indicates Osteoclast. Scale bars, 100 μm (low field), 50 μm (high field). f The analysis of the number of osteoclasts. g Immunohistochemistry of VEGF in the femoral head of different groups. Scale bars, 100 μm (low field), 50 μm (high field). h Quantification of immunohistochemical analysis of the VEGF level of each group. i, j The representative images of CD31 stain of different indicated processing groups. The red arrow indicates blood vessels. Scale bars, 100 μm (low field), 50 μm (high field). (n = 5 per group, data are shown as mean ± SD, ***p < 0.001, **p < 0.01, *p < 0.05).

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