Fig. 3: ENG and VEGFR2 form stable complexes which are enhanced by VEGF-A.
From: NRP1 interacts with endoglin and VEGFR2 to modulate VEGF signaling and endothelial cell sprouting
COS7 cells were co-transfected with expression vectors encoding myc-VEGFR2 alone or together with HA-ENG (or empty vector) as in Fig. 2. Where shown, HA-ENG was immobilized by IgG-crosslinking as in Fig. 2. The lateral mobility of Fab’-labeled myc-VEGFR2 was measured by FRAP. Where indicated, BMP9 (5 ng/ml) or VEGF-A (50 ng/ml) were added during the last fluorescent labeling step for the FRAP experiment, and maintained thereafter. Representative FRAP curves of the lateral diffusion of myc-VEGFR2 coexpressed with uncrosslinked (a) or IgG-immobilized HA-ENG (b). Average Rf (c) and D values (d) showing the effect of coexpression and immobilization of HA-ENG (IgG crosslinking; CL) on the lateral diffusion of myc-VEGFR2. The bars depict the average values (mean ± SEM); the number of measurements (each conducted on a different cell) is shown under each bar. Asterisks indicate significant differences between the Rf values of the pairs indicated by brackets (*p < 0.05; **p < 0.01; ****p < 10−4; one-way ANOVA and Bonferroni post-hoc test. ns = not significant). A similar analysis of the D values showed no significant differences.
