Fig. 5: NRP1 overexpression in the presence of ENG enhances VEGF-A-mediated signaling in MEECs.
From: NRP1 interacts with endoglin and VEGFR2 to modulate VEGF signaling and endothelial cell sprouting
MEEC+/+ and MEEC-/- cells were transfected with myc-NRP1 or empty vector (control) as described under Methods. After 24 h, they were serum starved (30 min) followed by stimulation (5 min) with 50 ng/ml VEGF-A. Cell lysates were subjected to SDS-PAGE and immunoblotted for pVEGFR2, tVEGFR2, NRP1, pErk1/2, tErk1/2 and β-actin. a Representative immunoblot. Quantification of the effect of NRP1 overexpression on VEGF-A signaling to pVEGFR2 (b) or to pErk1/2 (c). The bands were visualized by ECL and quantified by densitometry. Data are mean ± SEM of 6−8 independent experiments. The values obtained for VEGF-A-stimulated MEEC+/+ transfected with empty vector were taken as 100%. Asterisks indicate significant differences between pairs of MEEC+/+ or MEEC-/- cells with or without myc-NRP1 overexpression (one-way ANOVA and Bonferroni post-hoc test; *p < 0.05; ***p < 10-3; ****p < 10-4). ns = not significant. The band marked by # in the representative blot (a) is non specific.
