Fig. 6: NRP1 knockdown inhibits VEGF-A signaling in MEECs expressing ENG.
From: NRP1 interacts with endoglin and VEGFR2 to modulate VEGF signaling and endothelial cell sprouting
MEEC+/+ and MEEC-/- cells were transfected with siRNA to NRP1 or scrambled siRNA (siScrm; control). After 48 h, conditions which effectively downregulated the mRNA levels of NRP1 (Supplementary Fig. 8), they were taken for signaling studies conducted as described in Fig. 5. a A representative immunoblot. Quantification of the effect of siNRP1 on VEGF-A signaling to pVEGFR2 (b) or to pErk1/2 (c). The bands were visualized by ECL and quantified by densitometry. Data are mean ± SEM of 4−5 independent experiments. The values obtained for VEGF-A-stimulated MEEC+/+ transfected with siScrm were taken as 100%. Asterisks indicate significant differences between pairs of MEEC+/+ or MEEC-/- cells with or without siNRP1 treatment (one-way ANOVA and Bonferroni post-hoc test; *p < 0.05; **p < 0.01; ***p < 10-3). ns = not significant. The band marked by # in the representative blot (a) is non specific.
