Fig. 1: HNF4A loss leads to reduced histone modifications at HBRs in the mouse liver.
From: HNF4A guides the MLL4 complex to establish and maintain H3K4me1 at gene regulatory elements
a Heatmap displaying histone modifications (H3K4me1 and H3K27ac) and chromatin accessibility (ATAC-seq) at HNF4A bound regions (HBRs) in control and cKO livers. A 5 kb window is shown with the summit of HNF4A binding in the centre of each panel. Regions with high levels of each TF, histone modification or accessibility are red while low signal is represented by green. b Average profile plots are shown with the HBRs being at the centre. Control liver is in blue while Hnf4a cKO liver is in red. c Genome browser view of HNF4A target gene Ido2 shows an example gene with reduced H3K4me1 (blue), H3K27ac (orange) and ATAC-seq (pink) in the cKO liver around the HNF4A bound region (green, highlighted). d RNA-seq data for genes associated with an HNF4A bound region show reduced overall expression in cKO livers (red) compared to control livers (blue). Mean is represented by black line. Significance was tested using a t test, ****p-value < 0.0001. n = 3 biologically independent samples.
