Fig. 1: Establishment of anti-DNA scFvs.
From: Molecular and structural basis of anti-DNA antibody specificity for pyrrolated proteins
a Schematic representation of lysine Nε-pyrrolation in proteins (left) and dual-specificity of anti-DNA Abs for DNA and pyrP (right). b Scheme for the establishment of anti-DNA scFvs from SLE-prone mice. c, Bio-panning selection of anti-DNA scFvs. The selection was evaluated by ELISA against sermon sperm genomic DNA using 1 × 1010 pfu polyclonal phage. Data are mean ± S.D. of triplicate samples (representative of three independent experiments). Student’s t-test (two-sided). d Screening of anti-DNA scFv. The binding capacity to DNA was evaluated by ELISA using monoclonal scFv-G3P from the bacterial cultures of individual clones. Screenings (left, first screening; right, second screening) were performed twice with 10 clones each. Data are mean ± S.D. of triplicate samples (representative of three independent experiments). Student’s t-test (two-sided), *p < 0.05; **p < 0.01; ***p < 0.001.
